Jim F Huggett
Jim F Huggett
National Measurement Laboratory/University of Surrey
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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ...
Clinical chemistry 55 (4), 611-622, 2009
Real-time RT-PCR normalisation; strategies and considerations
J Huggett, K Dheda, S Bustin, A Zumla
Genes & Immunity 6 (4), 279-284, 2005
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
K Dheda, JF Huggett, SA Bustin, MA Johnson, G Rook, A Zumla
Biotechniques 37 (1), 112-119, 2004
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ...
Clinical chemistry 59 (6), 892-902, 2013
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
K Dheda, JF Huggett, JS Chang, LU Kim, SA Bustin, MA Johnson, ...
Analytical biochemistry 344 (1), 141-143, 2005
MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
SA Bustin, JF Beaulieu, J Huggett, R Jaggi, FSB Kibenge, PA Olsvik, ...
BMC molecular biology 11, 1-5, 2010
Four-month moxifloxacin-based regimens for drug-sensitive tuberculosis
SH Gillespie, AM Crook, TD McHugh, CM Mendel, SK Meredith, ...
New England Journal of Medicine 371 (17), 1577-1587, 2014
Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
AS Whale, JF Huggett, S Cowen, V Speirs, J Shaw, S Ellison, CA Foy, ...
Nucleic acids research 40 (11), e82-e82, 2012
Considerations for digital PCR as an accurate molecular diagnostic tool
JF Huggett, S Cowen, CA Foy
Clinical chemistry 61 (1), 79-88, 2015
Evaluation of digital PCR for absolute DNA quantification
R Sanders, JF Huggett, CA Bushell, S Cowen, DJ Scott, CA Foy
Analytical chemistry 83 (17), 6474-6484, 2011
Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification
AS Devonshire, AS Whale, A Gutteridge, G Jones, S Cowen, CA Foy, ...
Analytical and bioanalytical chemistry 406, 6499-6512, 2014
Lung remodeling in pulmonary tuberculosis
K Dheda, H Booth, JF Huggett, MA Johnson, A Zumla, GAW Rook
The Journal of infectious diseases 192 (7), 1201-1210, 2005
The need for transparency and good practices in the qPCR literature
SA Bustin, V Benes, J Garson, J Hellemans, J Huggett, M Kubista, ...
Nature methods 10 (11), 1063-1067, 2013
Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon
JF Huggett, T Novak, JA Garson, C Green, SD Morris-Jones, RF Miller, ...
BMC research notes 1, 1-9, 2008
Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study
P Tissari, A Zumla, E Tarkka, S Mero, L Savolainen, M Vaara, A Aittakorpi, ...
The Lancet 375 (9710), 224-230, 2010
qPCR primer design revisited
S Bustin, J Huggett
Biomolecular detection and quantification 14, 19-28, 2017
Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs
JE Phelan, DM O’sullivan, D Machado, J Ramos, YEA Oppong, ...
Genome medicine 11, 1-7, 2019
The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020
JF Huggett
Clinical chemistry 66 (8), 1012-1029, 2020
Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay—a clinical validation study
A Rachow, A Zumla, N Heinrich, G Rojas-Ponce, B Mtafya, K Reither, ...
PloS one 6 (6), e20458, 2011
Fundamentals of multiplexing with digital PCR
AS Whale, JF Huggett, S Tzonev
Biomolecular detection and quantification 10, 15-23, 2016
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